Defined substrate technology test for E. coli
نویسندگان
چکیده
منابع مشابه
Enumeration of total coliforms and Escherichia coli from source water by the defined substrate technology.
Many water utilities are required to monitor source water for the presence of total coliforms, fecal coliforms, or both. The Colilert system, an application of the defined substrate technology, simultaneously detects the presence of both total coliforms and Escherichia coli directly from a water sample. After incubation, the formula becomes yellow if total coliforms are present and fluorescent ...
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The biochemical effects of CHIP have been studied in C3H mice with and without transplanted mammary tumour. The maximum tolerated dose of CHIP was first determined by lethality and intestinal crypt assays to be 40mgkg-' and this dose was used to assay the time course of gastric distension and the pattern of drug distribution. A high level of CHIP uptake was found in liver as well as kidney. For...
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We have developed a new litmus paper test (DipTest) for detecting Escherichia coli (E. coli) in water samples by performing enzymatic reactions directly on the porous paper substrate. The paper strip consists of a long narrow piece of cellulose blotting paper coated with chemoattractant (at bottom edge), wax hydrophobic barrier (at the top edge), and custom formulated chemical reagents (at reac...
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Two commercially available defined-substrate coliform tests were compared with EC medium plus 4-methylumbelliferyl-p-D-glucuronide (MUG) for detecting chlorine-exposed Escherichia coli in spiked water samples. Statistical analyses of the test results indicated no sign!nifcant differences in detection of E. cofi between the Autoanalysis Colilert test and EC medium with MUG. There were, however, ...
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The hydrolysis of several phenylacetylamino compounds was studied using a purified preparation of E. coli penicillin acylase. The L-isomers of phenylacetyl amino acids were cleaved much faster than the D-isomers. The same observations was made for some phenylacetylamino beta-lactams. When the beta-lactam ring is incorporated in a penam or cephem ring system, the D-isomers were hydrolysed somewh...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1991
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.57.11.3403-3404.1991